Silambarasan Kanesan — four and a half years spanning molecular biology and biochemistry, from PCR-based diagnostics to UV-Vis spectroscopy, now working toward the thing I actually want: hands-on research in molecular biophysics — in a PhD program or at a company doing that work.
I've spent four and a half years inside molecular diagnostics R&D — developing and validating Laboratory Developed Tests, working with DNA/RNA extraction chemistry, and operating inside ISO 15189:2022 and ISO/IEC 17025:2017 quality systems. It's precise, regulated, unglamorous work, and it taught me how to validate a method properly — a discipline that transfers directly to research.
That's not the whole picture, though. Before the industry role, my B.Sc. and M.Sc. dissertations gave me my first real exposure to independent research — spectrophotometric assays, chromatography, and enzyme inhibition kinetics, applied to plant biochemistry rather than diagnostics. It's not biophysics, but it's where I first learned to design and run an experiment on my own, not just execute someone else's protocol.
What I still don't have is the thing I actually want: an understanding of biomolecular structure and mechanism at the biophysical level. That's the gap this site is built around, not the one it pretends doesn't exist.
What draws me to this field specifically is a fascination with mechanism at the smallest scale — how molecular and physical processes underlie the difference between health and disease, and how a body forms correctly in the first place. Even my DPPH antioxidant assay work during my M.Sc. was, at its core, measuring electrons absorbing and releasing light — a small, early example of the kind of physical measurement I want to spend a career getting properly trained in. It's a broad curiosity right now, not a finished research question — the work ahead is turning it into one.
DNA/RNA extraction (incl. low-biomass, cfDNA/cfRNA, microbiome, degraded/plant samples) • Direct PCR • PCR/qPCR/RT-PCR/RT-qPCR • gene expression analysis • GMO detection & screening • assay development & optimization • analytical/method validation • clinical & food molecular diagnostics
ISO 15189:2022 • ISO/IEC 17025:2017 • NABL accreditation support • SOP preparation • technical documentation
Patent documentation (Abstract, Specification, Drawings, Claims) • USPTO / IPO application follow-up
UV-Vis spectrophotometry (cuvette & microplate reader) • Total Polyphenols assay • antioxidant assay • MDA (malondialdehyde) assay • secondary metabolite extraction & estimation • phytochemical screening • thin-layer chromatography (TLC) • DPPH antioxidant assay • enzyme inhibition assay
Currently building Python proficiency for scientific/biophysical data analysis (self-directed, 2026–). Basic familiarity with HTML/MySQL from unrelated coursework.
Note: No prior formal training in structural biology or biophysical instrumentation (e.g., X-ray, cryo-EM, NMR, SPR, ITC, CD) — this is the current focus of active skill-building.
Investigated the antidiabetic and antioxidant potential of an ethanolic extract of Solanum trilobatum, a plant used in traditional Ayurvedic and Siddha medicine. Standardized the plant material, screened it for phytochemical constituents, and quantified key secondary metabolites. Assessed antioxidant activity via the DPPH radical scavenging assay, and antidiabetic potential via α-amylase enzyme inhibition (benchmarked against acarbose) and an in vitro glucose diffusion assay. The extract showed measurable antioxidant activity and statistically significant (p<0.05) glucose diffusion retardation compared to control — coursework-level pharmacognosy research, not a peer-reviewed publication.
Mimosa pudica leaves were shade-dried and powdered, then extracted using methanol to isolate secondary metabolites. The extract was screened for phytochemical constituents and analyzed by thin-layer chromatography (TLC) to separate and identify individual components. Antioxidant activity was assessed using the DPPH radical scavenging assay. First research project, undertaken as part of undergraduate coursework.
Open to research assistantships, technician roles, industry R&D positions, and funded master's or fellowship opportunities in molecular biophysics and structural biology — academic or company setting.