Molecular Biophysics · In Transition

From diagnostics
to structure.

Silambarasan Kanesan — four and a half years spanning molecular biology and biochemistry, from PCR-based diagnostics to UV-Vis spectroscopy, now working toward the thing I actually want: hands-on research in molecular biophysics — in a PhD program or at a company doing that work.

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A researcher built in the lab, now reaching for the bench beside it

Portrait of Silambarasan Kanesan

I've spent four and a half years inside molecular diagnostics R&D — developing and validating Laboratory Developed Tests, working with DNA/RNA extraction chemistry, and operating inside ISO 15189:2022 and ISO/IEC 17025:2017 quality systems. It's precise, regulated, unglamorous work, and it taught me how to validate a method properly — a discipline that transfers directly to research.

That's not the whole picture, though. Before the industry role, my B.Sc. and M.Sc. dissertations gave me my first real exposure to independent research — spectrophotometric assays, chromatography, and enzyme inhibition kinetics, applied to plant biochemistry rather than diagnostics. It's not biophysics, but it's where I first learned to design and run an experiment on my own, not just execute someone else's protocol.

What I still don't have is the thing I actually want: an understanding of biomolecular structure and mechanism at the biophysical level. That's the gap this site is built around, not the one it pretends doesn't exist.

What draws me to this field specifically is a fascination with mechanism at the smallest scale — how molecular and physical processes underlie the difference between health and disease, and how a body forms correctly in the first place. Even my DPPH antioxidant assay work during my M.Sc. was, at its core, measuring electrons absorbing and releasing light — a small, early example of the kind of physical measurement I want to spend a career getting properly trained in. It's a broad curiosity right now, not a finished research question — the work ahead is turning it into one.

Research Interests
Molecular Biophysics Structural Biology Spectroscopy & Biomolecular Interactions Molecular Diagnostics
Currently Learning
  • Python for scientific and biophysical data analysis — self-directed, started 2026
  • Foundational spectroscopy and structural biophysics concepts (UV-Vis, CD, X-ray, cryo-EM) — self-study, ongoing
This is a transition in progress, documented honestly — established diagnostics experience on one side, biophysics training I'm actively building on the other.
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Trajectory

Next
Research role in molecular/structural biophysics — PhD, research master's, fellowship, lab technician, or industry R&D position
Target — open to relocation, academic or company setting
Dec 2021 — Present
Research Assistant, Molecular Diagnostics R&D
AMMAGENOMICS LLP, Chennai, India
  • Molecular Biology — LDT development; DNA/RNA extraction across degraded plant, viral, microbiome (soil/stool/saliva), low-biomass, and cell-free (cfDNA/cfRNA) samples; PCR/qPCR/RT-PCR/RT-qPCR for COVID-19 diagnostics, point mutation detection, and gene expression analysis (mitochondrial RNA, immune marker, cancer marker); Direct PCR; GMO detection and screening assays
  • Biochemistry — UV-Vis spectrophotometry (cuvette & microplate reader): Total Polyphenols, antioxidant, and MDA assays; LOD/LOQ-based assay validation
  • Quality & IP — ISO 15189:2022 / ISO 17025:2017 systems, NABL accreditation support; patent documentation (Abstract, Specification, Drawings, Claims), USPTO/IPO filing tracking
  • Digital Workflow — defined lab-side requirements for an AI-assisted test workflow application
2018 — 2020
M.Sc. Biochemistry
Srimad Andavan Arts and Science College · Bharathidasan University
  • Dissertation: phytochemical screening and antidiabetic/antioxidant assays on Solanum trilobatum extract — see Education for detail
2015 — 2018
B.Sc. Biochemistry, First Class
Srimad Andavan Arts and Science College · Bharathidasan University
  • Dissertation: phytochemical screening and antioxidant assay on Mimosa pudica extract — see Education for detail
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Technical Skills

Molecular Biology & Diagnostics

DNA/RNA extraction (incl. low-biomass, cfDNA/cfRNA, microbiome, degraded/plant samples) Direct PCR PCR/qPCR/RT-PCR/RT-qPCR gene expression analysis GMO detection & screening assay development & optimization analytical/method validation clinical & food molecular diagnostics

Laboratory Quality Systems

ISO 15189:2022 ISO/IEC 17025:2017 NABL accreditation support SOP preparation technical documentation

Intellectual Property

Patent documentation (Abstract, Specification, Drawings, Claims) USPTO / IPO application follow-up

Analytical & Spectroscopic Techniques

UV-Vis spectrophotometry (cuvette & microplate reader) Total Polyphenols assay antioxidant assay MDA (malondialdehyde) assay secondary metabolite extraction & estimation phytochemical screening thin-layer chromatography (TLC) DPPH antioxidant assay enzyme inhibition assay

Computational Skills (in progress)

Currently building Python proficiency for scientific/biophysical data analysis (self-directed, 2026–). Basic familiarity with HTML/MySQL from unrelated coursework.

Note: No prior formal training in structural biology or biophysical instrumentation (e.g., X-ray, cryo-EM, NMR, SPR, ITC, CD) — this is the current focus of active skill-building.

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Education

M.Sc. Biochemistry
Srimad Andavan Arts and Science College (Autonomous), Bharathidasan University
2018 — 2020
M.Sc. Dissertation Project
Preliminary Phytochemical Screening, In Vitro Antidiabetic and Antioxidant Study of Solanum trilobatum Linn.
Supervisor: Mr. G. Hariharan, M.Sc., M.Phil. · Department of Biochemistry · Submitted April 2020

Investigated the antidiabetic and antioxidant potential of an ethanolic extract of Solanum trilobatum, a plant used in traditional Ayurvedic and Siddha medicine. Standardized the plant material, screened it for phytochemical constituents, and quantified key secondary metabolites. Assessed antioxidant activity via the DPPH radical scavenging assay, and antidiabetic potential via α-amylase enzyme inhibition (benchmarked against acarbose) and an in vitro glucose diffusion assay. The extract showed measurable antioxidant activity and statistically significant (p<0.05) glucose diffusion retardation compared to control — coursework-level pharmacognosy research, not a peer-reviewed publication.

Solvent extraction (Soxhlet) Phytochemical screening Spectrophotometric assays DPPH antioxidant assay Enzyme inhibition assay Statistical comparison to standard drug
B.Sc. Biochemistry — First Class
Srimad Andavan Arts and Science College (Autonomous), Bharathidasan University
2015 — 2018
B.Sc. Dissertation Project
Phytochemical Analysis and Screening, In Vitro Antioxidant Potential of Methanolic Extract of Mimosa pudica
Department of Biochemistry · 2017 — 2018

Mimosa pudica leaves were shade-dried and powdered, then extracted using methanol to isolate secondary metabolites. The extract was screened for phytochemical constituents and analyzed by thin-layer chromatography (TLC) to separate and identify individual components. Antioxidant activity was assessed using the DPPH radical scavenging assay. First research project, undertaken as part of undergraduate coursework.

Sample preparation (shade-drying, grinding) Methanolic extraction Phytochemical screening Thin-layer chromatography (TLC) DPPH antioxidant assay
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Let's talk research.

Open to research assistantships, technician roles, industry R&D positions, and funded master's or fellowship opportunities in molecular biophysics and structural biology — academic or company setting.